Journal: Cytoskeleton (Hoboken, N.j.)

Article Title: ARC Expands the DAAM1 Microexon‐Mediated Actin– RHOA / ROCK Interplay

doi: 10.1002/cm.22051

Figure Lengend Snippet: ARC protein expands the feedback loop between Actin, RHOA/ROCK signaling cascade, and the DAAM1 microexon. (a) Representative immunocytochemistry assay images of mature glutamatergic neurons (day in vitro 21; DIV21), stained with neuronal morphology marker β3‐Tubulin (green) and IEG‐positive nuclei for ARC (magenta), cFOS (red), and EGR1 (cyan). (b) Percentage quantification of ARC, cFOS, and EGR1‐positive neuronal nuclei normalized to their total number based on DAPI (not shown). One dot represents one cell line average, for which 3–4 randomly selected regions of interest were analyzed. Different point shapes represent biological replicates. p values from a two‐way ANOVA test with replicate and genotype as factors. (c) Representative images of ARC immunohistochemistry (green) and DAPI‐stained nuclei (blue) in sections of the dentate gyrus of the hippocampus from PD21 mice. (d,e) Quantifications of IEG‐positive nuclei in the hippocampal dentate gyrus across images normalized to the dentate gyrus size for ARC in basal conditions (d), or 1.5 h after learning (e) with ARC (left panel) and cFOS (right panel). p values from two‐sided Wilcoxon rank‐sum tests. One dot represents one animal for which 3–6 coronal views of the hippocampus were analyzed. (f) Overview of the phenotypes observed following the removal of Daam1‐MIC and their connection to ARC and the RHOA/ROCK signaling pathway (Habas et al. ; Liu et al. ; Schönichen and Geyer ). DVL interacts with DAAM1, which removes its autoinhibition. This is further enhanced by additional interactions with RHOA. Active DAAM1 triggers RHOA activation via an unclear mechanism linked to DAAM1's C‐terminal region (dashed line) and does not rely on direct binding between RHOA and DAAM1. Microexon removal reduces RHOA binding to DAAM1's N‐terminal region. This is proposed to lead to reduced hydrolysis of RhoA‐GTP, which increases the pool of active RHOA and, in turn, hyperactivates the RHOA/ROCK signaling cascade. Impaired actin cytoskeleton presumably enhances local translation of ARC mRNA and increases ARC protein levels. Arrows show the direction of the event. The dotted arrow suggests a potential, unknown feedback loop involving actin polymerization, ARC levels, and RHOA/ROCK pathway activation. ARC, Activity‐regulated cytoskeleton‐associated protein; GAP, GTPase‐activating protein; GEF, Guanine nucleotide exchange factor; GTP, Guanosine triphosphate; GDP, Guanosine diphosphate.

Article Snippet: “ ARC Expands the DAAM1 Microexon‐Mediated Actin– RHOA / ROCK Interplay .” Cytoskeleton 83 , no. 3 : 123 – 127 .

Techniques: Immunocytochemistry, In Vitro, Staining, Marker, Immunohistochemistry, Activation Assay, Binding Assay, Activity Assay

Journal: Clinical and Translational Medicine

Article Title: Uterus globulin associated protein 1 (UGRP1) binds podoplanin (PDPN) to promote a novel inflammation pathway during Streptococcus pneumoniae infection

doi: 10.1002/ctm2.850

Figure Lengend Snippet:

Article Snippet: Active Rho Detection Kit , Cell Signaling Technology , Cat# 8820S.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Cell Counting, Extraction, Software, Real-time Polymerase Chain Reaction